An Integrated System for Anaerobic Structural Genomics

Oxygen-sensitive proteins are targets for the treatment of cancer, HIV, Herpes, Anthrax and Tuberculosis. They also control the global food chains and offer environmental scientists new strategies for biofuel development and bioremediation. Equally important, the very considerable Bio-inorganic community has not significantly benefited from a structural genomics type approach due to the lack of appropriate infrastructure. To address these challenges, we have assembled a unique instrumentation system, including approximately 50 linear feet of glove box space (shown below), which has allowed for the recapitulation of our established structural genomics pipeline in an anaerobic environment to facilitate high-throughput protein expression, purification, crystallization and structure determination of oxygen-sensitive proteins. The materials arising from these efforts (verified expression clones and purified proteins) will also support a wide range of functional, mechanistic and systems biology studies, and will be freely distributed. We will actively solicit proposals from the community to process targets of interest for users and invite users to visit NYSGRC (http://www.nysgxrc.org) in order to directly utilize this system and more fully integrate with the PSI:Biology Network (http://sbkb.org)
Please contact Dr James Love for more details: james.love@einstein.yu.edu

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